Regulatory Effects of Thai Rice By-Product Extracts from Oryza sativa L. cv. Bue Bang 3 CMU and Bue Bang 4 CMU on Melanin Production, Nitric Oxide Secretion, and Steroid 5α-Reductase Inhibition.
Warintorn Ruksiriwanich,
Pichchapa Linsaenkart,
Chiranan Khantham,
Anurak Muangsanguan,
Korawan Sringarm
et al.
Other
Plants (Basel, Switzerland)
2023
14 citazioni
PubMed
DOI
CC-BY
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Study Design
- Tipo di studio
- in vitro study
- Intervento
- Regulatory Effects of Thai Rice By-Product Extracts from Oryza sativa L. cv. Bue Bang 3 CMU and Bue Bang 4 CMU on Melanin Production, Nitric Oxide Secretion, and Steroid 5α-Reductase Inhibition. not specified
- Comparatore
- Placebo
- Direzione dell'effetto
- Positive
- Rischio di bias
- High
Abstract
Alopecia and gray hair are common hair abnormalities affecting physical appearance and causing psychological problems. Chemical treatments partially restore hair disorders but have distressing side effects. Bioactive plant compounds constitute promising sources of potential medicinal substances instead of chemical agents, producing high side effects. In this study, we focused on the waste of local rice cultivars: Bue Bang 3 CMU (BB3CMU) and Bue Bang 4 CMU (BB4CMU) from the north of Thailand. The rice bran oil (RBO), defatted rice bran extract (DFRB), and rice husk (H) were determined for in vitro hair revitalization in melanin production, nitric oxide (NO) secretion, and steroid 5α-reductase inhibition. The results indicated that BB4CMU-RBO with high contents of iron, zinc, and free fatty acids showed a comparable induction of melanin production on melanocytes (130.18 ± 9.13% of control) to the standard drug theophylline with no significant difference (p > 0.05). This promising melanin induction could be related to activating the NO secretion pathway, with the NO secretion level at 1.43 ± 0.05 µM. In addition, BB4CMU-RBO illustrated a significant inhibitory effect on both steroid 5α-reductase genes (SRD5A) type 1 and type 2, which relates to its primary source of tocopherols. Hence, rice bran oil from the Thai rice variety BB4CMU could be applied as a promising hair revitalizing candidate, from natural resources, to help promote hair growth and re-pigmentation effects.
Full Text
Figures
Figure 1
Cell viability assays of melanocyte and dermal papilla cells treated with Thai rice by-product extracts (Bue Bang 3 CMU and Bue Bang 4 CMU) demonstrate the non-toxic concentration ranges suitable for subsequent functional studies.
chart
Figure 2
Melanin content quantification in B16F10 melanoma cells treated with rice by-product extracts shows dose-dependent effects on pigmentation, suggesting potential regulatory activity on melanogenesis pathways.
chart
Figure 3
Tyrosinase activity measurements in melanocyte cultures exposed to Thai rice extracts indicate modulation of the key enzyme in melanin biosynthesis. The results suggest both stimulatory and inhibitory effects depending on extract variety and concentration.
chart
Figure 4
Dermal papilla cell proliferation and hair growth-related gene expression analysis following treatment with rice bran extracts reveal upregulation of growth factors associated with hair follicle cycling.
chartTables
Table 1
| Extracts | DPPH Radical Scavenging Activity | ABTS Radical Scavenging Activity | Iron Chelating Activity |
|---|---|---|---|
| BB3CMU−RBO | 70.14 ± 0.97 a | 5.45 ± 0.28 a | 28.79 ± 5.63 a |
| BB3CMU−DFRB | 147.65 ± 2.05 b | 51.83 ± 2.67 b | 65.47 ± 12.79 a |
| BB3CMU−H | 334.70 ± 4.64 c | 196.13 ± 10.09 c | 283.22 ± 55.35 b |
| BB4CMU−RBO | 37.05 ± 0.51 d | 3.84 ± 0.20 a | 24.28 ± 4.74 a |
| BB4CMU−DFRB | 117.84 ± 1.63 e | 47.03 ± 2.42 b | 71.78 ± 14.03 a |
| BB4CMU−H | 198.04 ± 2.75 f | 164.10 ± 8.45 d | 61.12 ± 11.94 a |
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