Investigating the potential mechanism of microneedling in alopecia areata mice based on 16S rRNA sequencing and metabolomics.
Study Design
- Type d'étude
- animal study
- Durée
- 2 weeks
- Intervention
- Investigating the potential mechanism of microneedling in alopecia areata mice based on 16S rRNA sequencing and metabolomics. Halometasone topical applied every 2 days for 2 weeks after hand-held dermal microneedling treatment
- Comparateur
- Placebo
- Direction de l'effet
- Positive
- Risque de biais
- Unclear
Abstract
BACKGROUND: The present study investigates the relationship between alopecia areata (AA) and intestinal microecology, examining the effect of microneedling on the microecology of alopecia areata. METHODS: An animal model of AA was established using imiquimod-induced C3H/HeJ mice. Halometasone was applied topically every 2 days for 2 weeks after a hand-held dermal microneedling treatment. Fecal samples were collected before and after the interventions and underwent 16S rRNA high-throughput sequencing to assess intestinal microecological alterations. Furthermore, changes in short-chain fatty acids (SCFA) associated with AA and microneedling treatment were analyzed. RESULTS: Microneedling therapy enhanced hair growth in the model group. The model group exhibited a substantial decline in diversity and abundance of gut microbes compared to the control group. After microneedling treatment, the diversity of intestinal microbes was restored, along with a concurrent remodeling of both pathogenic and beneficial bacterial compositions in the model group. In addition, the levels of acetic acid and propanoic acid were elevated in the model group compared to the control group. Following microneedling treatment, a reduction in these levels was observed. In contrast, the model group showed an increase in butanoic acid levels after microneedling treatment; however, this increase did not reach statistical significance. CONCLUSION: Microneedling treatment has been shown to improve hair regeneration in AA. Additionally, it positively affects the intestinal microecology related to AA, leading to changes in gut microbes and the production of SCFAs. This provides a foundation for the clinical application of microneedling treatment in AA.
Full Text
Figures
Figure 1
Experimental design for studying the effects of microneedling on intestinal microecology in alopecia areata mice using 16S rRNA sequencing and metabolomics.
diagram
Figure 2
16S rRNA sequencing results showing gut microbiota composition differences between alopecia areata model mice and controls, with changes after microneedling treatment.
chart
Figure 3
Alpha diversity indices comparing intestinal microbial richness and evenness across alopecia areata and microneedling-treated mouse groups.
chart
Figure 4
Beta diversity ordination plot visualizing distinct clustering of gut microbiota communities between alopecia areata and treated mice.
chart
Figure 5
Metabolomic profiling results showing differentially abundant metabolites in alopecia areata mice that shift following microneedling intervention.
chart
Figure 6
Correlation analysis linking specific gut bacteria to metabolic pathway changes in the alopecia areata microneedling study.
chart
Figure 7
Proposed mechanism diagram integrating microbiome and metabolome findings to explain how microneedling may influence alopecia areata through the gut-skin axis.
diagramReferences
- Common causes of hair loss—clinical manifestations, trichoscopy and therapy J. Eur. Acad. Dermatol. Venereol., 2021
- Growth factors and microneedling in alopecia areata: a narrative review Skin Appendage Disord., 2024
- Alopecia areata and the gut-the link opens up for novel therapeutic interventions Expert Opin. Ther. Targets, 2018
- Gut microbiota in alopecia areata Postepy Dermatol. Alergol., 2022
- Gut microbiota and type 1 diabetes mellitus: the effect of Mediterranean diet Front. Nutr., 2021
- Mucosal metabolomic signatures in chronic colitis: novel insights into the pathophysiology of inflammatory bowel disease Metabolites, 2023
- Parasutterella, in association with irritable bowel syndrome and intestinal chronic inflammation J. Gastroenterol. Hepatol., 2018
- The role of methanogenic archaea in inflammatory bowel disease—a review J. Pers. Med., 2024
- The gut commensal Bacteroides thetaiotaomicron exacerbates enteric infection through modification of the metabolic landscape Cell Host Microbe, 2014
- The epigenetic state of IL-4-polarized macrophages enables inflammatory cistromic expansion and extended synergistic response to TLR ligands Immunity, 2022
- The skin and gut microbiome and its role in common dermatologic conditions Microorganisms, 2019
- Microneedling and its use in hair loss disorders: a systematic review Dermatol. Ther., 2022
- Microneedling for the treatment of hair loss? J. Eur. Acad. Dermatol. Venereol., 2018
- The maternal gut microbiome during pregnancy and offspring allergy and asthma J. Allergy Clin. Immunol., 2021
- The role of probiotics in skin health and related gut-skin axis: a review Nutrients, 2023
- The human microbiome: our second genome Annu. Rev. Genomics Hum. Genet., 2012
- Gallstone disease, obesity and the Firmicutes/Bacteroidetes ratio as a possible biomarker of gut dysbiosis J. Pers. Med., 2020
- Exploring the differences in the gut microbiome in atopic dermatitis according to the presence of gastrointestinal symptoms J. Clin. Med., 2022
- Alopecia areata and risk of atopic and autoimmune conditions: population-based cohort study Clin. Exp. Dermatol., 2023
- Plasmacytoid dendritic cells as a possible key player to initiate alopecia areata in the C3H/HeJ mouse Allergol. Int., 2020
- Butyrate induced Tregs are capable of migration from the GALT to the pancreas to restore immunological tolerance during type-1 diabetes Sci. Rep., 2020
- The causality between gut microbes and allergic diseases: insights from a bi-directional two-sample Mendelian randomization analysis Front. Immunol., 2023
- Assessment of bacterial diversity in breast milk using culture-dependent and culture-independent approaches Br. J. Nutr., 2013
- Free fatty acid receptors in health and disease Physiol. Rev., 2020
- Generation of regulatory dendritic cells and CD4+ Foxp3+ T cells by probiotics administration suppresses immune disorders Proc. Natl. Acad. Sci. U.S.A., 2010
- Pro- and anti-inflammatory effects of short chain fatty acids on immune and endothelial cells Eur. J. Pharmacol., 2018
- Regulation of short-chain fatty acids in the immune system Front. Immunol., 2023
- Gut microbiota characterization in Chinese patients with alopecia areata J. Dermatol. Sci., 2021
- The association between alopecia and inflammatory bowel disease: a systematic review and meta-analysis Dermatology, 2021
- Effects of diet on the childhood gut microbiome and its implications for atopic dermatitis J. Allergy Clin. Immunol., 2019
- Impact of gut microbiome on skin health: gut-skin axis observed through the lenses of therapeutics and skin diseases Gut Microbes, 2022
- The Firmicutes/Bacteroidetes ratio of the human microbiota changes with age BMC Microbiol., 2009
- Gut commensal-induced IκBζ expression in dendritic cells influences the Th17 response Front. Immunol., 2021
- Analysis of the gut microbiota in alopecia areata: identification of bacterial biomarkers J. Eur. Acad. Dermatol. Venereol., 2020
- TH1 cell-inducing Escherichia coli strain identified from the small intestinal mucosa of patients with Crohn’s disease Gut Microbes, 2020
- Gut microbiota and psychiatric disorders: a two-sample Mendelian randomization study Front. Microbiol., 2022
- The gut-skin axis in health and disease: a paradigm with therapeutic implications Bioessays, 2016
- Propionic acid promotes the virulent phenotype of Crohn’s disease-associated adherent-invasive Escherichia coli Cell Rep., 2020
- How our microbiome influences the pathogenesis of alopecia areata Genes, 2022
- The molecular mechanism of natural products activating Wnt/β-catenin signaling pathway for improving hair loss Life, 2022
- Proteobacteria: microbial signature of dysbiosis in gut microbiota Trends Biotechnol., 2015
- Clinical potential of anti-inflammatory effects of Faecalibacterium prausnitzii and butyrate in inflammatory bowel disease Inflamm. Bowel Dis., 2019
- The microbial metabolites, short-chain fatty acids, regulate colonic Treg cell homeostasis Science, 2013
- The influence of probiotics on the Firmicutes/Bacteroidetes ratio in the treatment of obesity and inflammatory bowel disease Microorganisms, 2020
- Roseburia spp.: a marker of health? Future Microbiol., 2017
- Differences in the gut Firmicutes to Bacteroidetes ratio across age groups in healthy Ukrainian population BMC Microbiol., 2020
- Bacterial translocation is linked to increased intestinal IFN-γ, IL-4, IL-17, and mucin-2 in cholestatic rats Ann. Hepatol., 2018
- Microneedles: a smart approach and increasing potential for transdermal drug delivery system Biomed. Pharmacother., 2019
- Bacopaside I alleviates depressive-like behaviors by modulating the gut microbiome and host metabolism in CUMS-induced mice Biomed. Pharmacother., 2024
- The role of serum Th1, Th2, and Th17 cytokines in patients with alopecia areata: clinical implications Cells, 2021
- Extremely hair follicle density is associated with a significantly different cecal microbiota in rex rabbits Exp. Dermatol., 2023
- Changes and significance of Th1/Th2 and Treg/Th17 cells and their cytokines in patients with alopecia areata Exp. Cell Res., 2024
- Short-chain fatty acids in diseases Cell Commun. Signal, 2023
- Integrated analysis reveals important differences in the gut and oropharyngeal microbiota between children with mild and severe hand, foot, and mouth disease Emerg. Microbes Infect., 2023
- IL-18 maintains the homeostasis of mucosal immune system via inflammasome-independent but microbiota-dependent manner Sci. Bull., 2021
Used In Evidence Reviews
Similar Papers
International journal of trichology · 2013
A randomized evaluator blinded study of effect of microneedling in androgenetic alopecia: a pilot study.
Journal of plastic, reconstructive & aesthetic surgery : JPRAS · 2018
Microneedling: Where do we stand now? A systematic review of the literature.
Dermatologic therapy · 2019
Physiopathology and current treatments of androgenetic alopecia: Going beyond androgens and anti-androgens.
Journal of the European Academy of Dermatology and Venereology : JEADV · 2018
Microneedling for the treatment of hair loss?
Indian journal of dermatology · 2015
Response to Microneedling Treatment in Men with Androgenetic Alopecia Who Failed to Respond to Conventional Therapy.
Journal of cutaneous and aesthetic surgery · 2014